Histamine 1 and 4 receptors involved in intestinal allergic responses in mouse model of peanut allergy
WEDNESDAY, April 13, 2016 (HealthDay News) — Blockade of histamine 1 (H1R) and 4 receptors (H4R) in peanut challenge can combine to prevent development of intestinal allergic responses, according to an experimental study published online April 5 in Allergy.
Meiqin Wang, M.D., Ph.D., from National Jewish Health in Denver, and colleagues examined the role of H1R and H4R in intestinal allergic responses in Balb/c mice that were sensitized and challenged to peanut. Mice were treated orally with the H1R antagonist loratadine and/or the H4R antagonist JNJ7777120 during the challenge phase. Dendritic cells (DCs) derived from bone marrow were adoptively transferred to nonsensitized wild-type mice.
The researchers found that individual loratadine or JNJ7777120 treatment partially suppressed diarrhea development and intestinal inflammation and correlated with a reduction in the numbers of DCs in mesenteric lymph nodes and lamina propria. Development of diarrhea and intestinal inflammation were prevented with combined treatment with both drugs. In vitro, the combination suppressed DC antigen presenting cell function to T helper cells as well as DC calcium mobilization and chemotaxis to histamine.
“Blockade of both H1R and H4R in the challenge phase had additive effects in preventing the intestinal consequences of peanut sensitization and challenge,” the authors write. “These effects were mediated through limitation of mesenteric lymph node and intestinal DC accumulation and function.”
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